Extraction of lipase from waste fruit seeds and its biomedical applications

Abstract

Background: Lipases have widespread applications in the field of  leather and detergent industry. Aim: The aim of this study was the  production of lipase from various fruit seeds and its usage in detergent  industry and in laundry product for removing oil and fat stains.  Methods: In the present study three waste fruit seeds (water melon,  honeydew melon, and cantaloupe melon) were used for the extraction and partial purification of lipase. Initially extraction of crude enzyme was carried out and partial purification of lipase done by ammonium sulphate precipitation method. Both crude and purified lipase product were screened for lipolytic activity using tween 80. Purified extract of all the samples showed the maximum tween 80 hydrolysis compared to the crude extract. The antibacterial activity of crude and purified extracts were performed against six clinical pathogens (Escherichia coli, Pseudomonas aeruginosa, Streptococcus pyogenes, Klebsiella pneumonia, Staphylococcus aureus, and Staphylococcus epidermidis). Results: The purified extract of water melon showed maximum zone of inhibition against all the pathogens except Staphylococcus aureus and Klebsiella pneumonia. Purified extract of honeydew melon seeds had great antibacterial effect compared to crude extract. Purified extract showed maximum zone of inhibition against all the pathogens. Purified extract of cantaloupe melon showed maximum inhibition of all tested bacterial pathogens compared to crude extract. Similarly, lipolytic enzyme extracted from waste fruit seed were also efficiently involved in the removal of oil and fat stains. Conclusion: it was  concluded that extract of waste fruit seeds may serve as an antibacterial 
and destaining agents.